Composite

Part:BBa_K3590041:Design

Designed by: Barbora Hrnčířová   Group: iGEM20_Brno_Czech_Republic   (2020-10-19)


Enzyme MlrB derived from Sphyngopyxis sp. fused with a dockerin derived from Bacteroides cellulosolv


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1769
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1325


Design Notes

For cloning purposes, attaaGAATTC (containing EcoRI) was added to 5' end and tttAAGCTT (containing HindIII) was added to 3' end. On both ends of the sequence, there are several nucleotides that help the restriction enzymes to interact with the sequence.


Source

This is a synthetic part. The sources of each fragment can be found at the individual page of each Basic Part that is present in this Composite Part.

References